molecular biology papers

Genetic Variability and Population Structure within the Anopheles tessellatus complex (Theobald, 1901) in Indonesia using ITS2 nuclear and COI, COII mitochondrial sequences

This study utilizes ITS2, COI, and COII molecular markers to reveal that the *Anopheles tessellatus* complex in Indonesia comprises three distinct genetic lineages across five islands with high haplotype diversity and potential speciation in specific populations, highlighting the need for further research to clarify its role in malaria transmission.

Nurwidayati, A., Purwanto, H., Astuti, R. R. U. N. W. + 6 more2026-04-10📄 molecular biology

Impact of Fluorophore and Epitope Position on Destabilized Reporter Performance in C. elegans

This study reveals that the performance of destabilized fluorescent reporters in *C. elegans* is critically dependent on the specific combination of fluorophore and epitope tag, demonstrating that certain configurations (such as a 3xFLAG adjacent to a PEST sequence or the use of mStayGold) can severely compromise the ability to detect dynamic gene expression oscillations.

Jackson, A., Ragle, J. M., Ward, J. D.2026-04-10📄 molecular biology

Copper deficiency drives OXPHOS impairment and mitochondrial hyperfusion via MTCH2 in skeletal muscle

This study demonstrates that skeletal muscle-specific copper deficiency impairs mitochondrial respiration and induces pathological hyperfusion via the copper-binding protein MTCH2, leading to exercise intolerance and metabolic dysfunction that can be rescued by restoring copper levels.

Lee, Y.-S., Kim, H. S., Nguyen, P. L. + 12 more2026-04-09📄 molecular biology

RAStoERK: a reference interaction atlas for insights into signaling and disease

This study presents RAStoERK, a comprehensive reference interaction atlas that maps the interactomes of the RAS/RAF/MEK/ERK pathway components and their mutations to reveal paralog-specific roles, mutation-driven rewiring, and novel crosstalk with processes like mRNA metabolism and WNT signaling, thereby providing a foundational resource for understanding the pathway's integration into physiology and disease.

Vucak, G., Didusch, S., Cannizzaro, L. + 4 more2026-04-09📄 molecular biology

Disentangling mitochondrial copy number variation and PCR amplification bias in DNA metabarcoding

This study investigates the limitations of quantitative DNA metabarcoding by demonstrating that high variation in mitochondrial copy numbers and stable PCR amplification biases prevent accurate biomass estimation, ultimately revealing fundamental constraints and the need for new methodological approaches despite the development of a mathematical model to correct for these biases.

Wolany, L., Klinkenborg, K., Leese, F. + 1 more2026-04-09📄 molecular biology

Prion Protein Deficiency Results in Synaptic, Neural Network and Behavioral Alterations

This study demonstrates that the cellular prion protein (PrPC) is essential for the proper development, maturation, and function of neuronal networks, as its deficiency leads to impaired synaptic dynamics, altered expression of key synaptic proteins, and heightened fear responses in mice.

Burato, A., Di Clemente, A., Lodetti, C. + 12 more2026-04-09📄 molecular biology

Signal, noise, and sampling: How pool size and replication shape metabolomic inference

This study demonstrates that in metabolomic analyses of small organisms, both pool size and biological replication are critical, interdependent factors that jointly determine the detectability and stability of biological signals, where insufficient pooling or replication leads to a loss of true metabolic effects without increasing false discoveries.

Hubert, D. L., Porter, D. L., Robinson, R. D. + 4 more2026-04-09📄 molecular biology

LINC00205 acts as a multivalent scaffold promoting FUSP525L recruitment in Amyotrophic Lateral Sclerosis stress granules

This study identifies the long non-coding RNA LINC00205 as a critical multivalent scaffold that drives the pathological persistence of stress granules in ALS by directly recruiting mutant FUSP525L and specific mRNAs, thereby establishing a mechanism for modulating aberrant RNA-protein condensates.

Rea, J., Stortini, G., Santini, T. + 8 more2026-04-09📄 molecular biology

MuteFree: A novel AAV vector system featuring mutation-free ITRs

MuteFree is a novel AAV transfer plasmid system that eliminates ITR mutations through combinatorial optimization of the plasmid backbone and flanking sequences, thereby enabling high-fidelity, high-yield production of therapeutic AAV vectors compatible with GMP manufacturing.

Shi, S. J., Lin, Y., Fu, E. Z. + 8 more2026-04-09📄 molecular biology

A trans-piRNA network and transcriptional antagonism shape piRNA cluster function

This study reveals that Drosophila piRNA cluster function relies on a trans-generational piRNA network for biogenesis and is constrained by transcriptional antagonism from newly integrated transposons, necessitating a revision of the traditional transposon trap model.

Luo, Y., Siqueira de Oliveira, D., He, P. + 1 more2026-04-09📄 molecular biology

Measuring mtDNA turnover, synthesis, and supercoiling via selective bromodeoxyuridine incorporation

This paper presents a protocol utilizing selective bromodeoxyuridine incorporation followed by a Southwestern blot to measure mitochondrial DNA turnover, synthesis, and supercoiling.

Deng, J., Mohan, A., Shutt, T.2026-04-08📄 molecular biology

Decoupling AMPK from fatty acid synthesis allows maintenance of fitness late in life

This study demonstrates that in budding yeast, constitutive AMPK activity decoupled from fatty acid synthesis inhibition prevents senescence and preserves late-life fitness in all aging cells by resolving the metabolic conflict between mitochondrial Acetyl-CoA transport and lipid starvation.

Hadj-Moussa, H., Ulusan, M., Horkai, D. + 2 more2026-04-08📄 molecular biology

Optimised genome editing for precise DNA insertion and substitution using Prime Editors in zebrafish

This study demonstrates that both nickase-based and nuclease-based Prime Editors can efficiently achieve precise DNA insertion and substitution in zebrafish without exogenous donor DNA, with the nuclease variant showing superior efficiency for short modifications and the ability to transmit these edits to the next generation.

Ono, Y., Peterka, M., Love, M. + 11 more2026-04-08📄 molecular biology

FlashBind: Towards Accurate and Efficient Structure-based Virtual Screening

FlashBind is a lightweight, structure-based virtual screening model that achieves a 50x speedup over the accurate but computationally expensive Boltz-2 by utilizing a fast docking model and streamlined EGNN architecture, while maintaining high predictive accuracy and demonstrating superior real-world efficacy in identifying potent antibiotic candidates against *E. coli*.

Jiang, S., Chen, Y., Krishnan, A. + 2 more2026-04-08📄 molecular biology

Protocol for in vivo DNA-RNA hybrid immunoprecipitation sequencing and analysis from frozen mammalian tissues

This paper presents a protocol for high-resolution, whole-genome mapping of DNA-RNA hybrids (R-loops) directly from frozen mammalian tissues using S9.6 antibody-based immunoprecipitation and sequencing.

Massalha, H., Chee, C. J., Mawer, J. S. P. + 2 more2026-04-08📄 molecular biology

Target RNA abundance controls the collateral activity of RfxCas13d in human cells and zebrafish embryos

This study reveals that the collateral RNA cleavage activity of RfxCas13d in human cells and zebrafish embryos is strictly threshold-dependent on target RNA abundance, where highly abundant targets trigger widespread toxicity and developmental defects, whereas moderately expressed targets allow for selective degradation, thereby highlighting the critical need to consider target levels when deploying this tool and suggesting PspCas13b as a safer alternative.

Chen, H., Hu, W., Impicciche, V. + 15 more2026-04-08📄 molecular biology

Mutations in apicoplast rRNA genes are associated with clindamycin resistance and impair the ability of malaria parasites to infect mosquitoes

This study demonstrates that clindamycin resistance in *Plasmodium falciparum* arises from mutations in apicoplast 23S rRNA genes, which, despite potentially impairing in vitro growth, do not completely block the parasite's ability to infect mosquitoes and spread in the field.

Home, J. L., Yeoh, L. M., McFadden, G. I. + 1 more2026-04-08📄 molecular biology

A luciferase-based assay for assessing IRES-mediated translation in Wheat Germ Extract

This paper describes a rapid and robust cell-free protocol using wheat germ extract and firefly luciferase reporters to quantitatively assess IRES-mediated translation and evaluate mRNA modifications designed to enhance translation efficiency.

Cortot, M., Stehlik, T., Koch, A. + 1 more2026-04-08📄 molecular biology

Longitudinal blood DNA methylation profiling reveals disrupted immune-epigenetic adaptation and candidate stress related loci in postpartum depression

This longitudinal study reveals that while healthy postpartum women exhibit a widespread, coordinated gain in immune-related DNA methylation as part of normative adaptation, women with postpartum depression display an attenuated and more heterogeneous epigenetic response, characterized by specific stress-related loci such as *FKBP5* and *AVP* that suggest disrupted immune-epigenetic adaptation contributes to postpartum vulnerability.

Wolff, P., Losse, E., Nehls, S. + 2 more2026-04-07📄 molecular biology

ZDHHC17 Links S-Acylation, Huntington Disease, VCP-associated Multisystem Proteinopathy, and Amyotrophic Lateral Sclerosis.

This study identifies ZDHHC17 as a critical S-acyltransferase that regulates the localization and toxicity of key proteins (including VCP and TDP-43) across multiple neurodegenerative diseases, revealing a shared mechanism of protein mislocalization driven by disrupted S-acylation.

Ramzan, F., Mishra, G. G., Alshehabi, Y. + 12 more2026-04-07📄 molecular biology

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